Mouse Cochlear Whole Mount Immunofluorescence
نویسندگان
چکیده
منابع مشابه
Mouse Cochlear Whole Mount Immunofluorescence.
This protocol comprises the entire process of immunofluorescence staining on mouse cochlea whole mount, starting from tissue preparation to the mounting of the tissue. This technique provides "three-dimensional" views of the stained components in order to determine the localization of a protein of interest in the tissue in its natural state and environment.
متن کاملWhole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea.
The organ of Corti, housed in the cochlea of the inner ear, contains mechanosensory hair cells and surrounding supporting cells which are organized in a spiral shape and have a tonotopic gradient for sound detection. The mouse cochlea is approximately 6 mm long and often divided into three turns (apex, middle, and base) for analysis. To investigate cell loss, cell division, or mosaic gene expre...
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PURPOSE To develop a new method of whole mount immunostaining that improves the penetration of staining reagents into the cornea and decreases non-specific binding and background. METHODS Adult mouse corneas were fixed overnight in 4% paraformaldehyde or a mixture of 4% paraformaldehyde and 0.2% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4, at 4 degrees C. After washing with 0.1% Triton X...
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INTRODUCTIONThe technique presented this protocol is ideal for obtaining an overall, three-dimensional picture of the distribution of an antigen in embryos from the prestreak stage to ~10.5 dpc (days post-coitum). The embryos are fixed and permeabilized so that antibodies can penetrate all of the tissues. However, antibodies will not penetrate the embryo completely if it is too large, and so it...
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The mouse is well-established as the major animal model for the study of mammalian development. Rapid progress in large-scale cDNA and also genomic sequencing projects is identifying new mouse genes at an unprecedented rate. As a first step toward understanding the function of these novel genes, it is important to determine their developmental expression pattern. Here we provide a reliable, sen...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2013
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.332